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Then, data from pH (total pH scale/mol kg −1 H 2 O), TA, measured salinity and temperature were used to estimate the parameters of the carbonate system speciation (partial pressure of CO 2 ( p CO 2 ), inorganic carbon (TIC), HCO −3 , , Ω aragonite and Ω calcite saturation states), using the CO2SYS program (Pierrot et al., 2.5 Sediment metal analysis The concentrations of metals (Cu, Pb, Zn, and Hg) in the sediments from each contamination treatment (control and metal contamination) were measured before the beginning of the experiments (T0) and at the end of exposure (T1) from each treatment
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