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Energetics of β-oxidation Each β-oxidation cycle can be represented as following: C (n) Acyl-CoA + CoA-SH + FAD + NAD + + H 2 O → C (n-2) Acyl CoA + Acetyl CoA + FADH 2 + NADH + H + Complete oxidation of Palmitoyl CoA can be represented as following: (Equation A) Palmitoyl CoA + 7CoA-SH + 7FAD + 7NAD + + 7H 2 O → 8Acetyl CoA + 7FADH 2 + 7NADH + 7H + Converting NADH and FADH 2 to their corresponding ATP equivalents from the above equation gives us: (Equation B) Palmitoyl CoA + 7CoA-SH + 7O 2 + 28P i + 28ADP → 8Acetyl CoA + 28ATP + 7H 2 O And after Acetyl CoA molecules enter Kreb’s cycle and Electron Transport Sytem, resulting ATP is shown below: 8Acetyl CoA + 16O 2 + 80P i + 80ADP → 8CoA + 80ATP + 16CO 2 + 16H 2 O Thus complete energy release is shown in the following equation by combining equation A and B: Palmitoyl CoA + 23O 2 + 108P i + 108ADP → CoA + 108ATP + 16CO 2 + 23H 2 O Note: There is utilization of ATP during the conversion of palmitic acid to palmitoyl-CoA and thus the net gain is ≈ 106 ATP