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The next day, media was removed and replaced with co-treatments as described: For glycolysis inhibition experiments, cells were pre-treated in glucose free RPMI 1640 with either vehicle, 300 nM KL-11743, 11 mM 2-DG, or 2 μM POMHEX for 20 minutes prior to spiking in RPMI 1640 equivalent concentration of U 13 C-glucose (11 mM)