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NAC (N-acetylcysteine) as precursor: Rather than supplementing glutathione directly, NAC provides the rate-limiting amino acid (cysteine) for endogenous glutathione synthesis

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Cells were then incubated with osteogenic induction medium: complete DMEM supplemented with 50 μg/mL vitamin C, 10 nmol/L dexamethasone and 10 mmol/L β-glycerophosphate (all from Sigma-Aldrich)