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Using these enzyme preparations with a defined GSH concentration (20 μM) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig
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[8] Absolute contraindications include prior hypersensitivity to cyanocobalamin, cobalt, or formulation excipients
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